酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Tyrosine Hydroxylase (TH) ,etc. by FLIA (Flow Luminescence Immunoassay)
TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14
（注：單次混測多因子不超過8個指標 ）
- 編號ULMB438Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.01-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.003 ng/mL.
- 樣本類型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-102 | 98 |
| EDTA plasma(n=5) | 87-97 | 92 |
| heparin plasma(n=5) | 89-101 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-104% | 81-102% | 78-95% | 99-105% |
| EDTA plasma(n=5) | 95-103% | 84-102% | 80-105% | 93-101% |
| heparin plasma(n=5) | 94-101% | 79-96% | 91-101% | 84-92% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
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相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPB438Ra01 | 酪氨酸羥化酶(TH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB438Ra01 | 酪氨酸羥化酶(TH)多克隆抗體 | WB; IHC |
| ULAB438Ra71 | 酪氨酸羥化酶(TH)多克隆抗體(生物素標記) | WB |
| UMAB438Ra25 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| UMAB438Ra21 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAB438Ra22 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| UMAB438Ra27 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| UMAB438Ra23 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| UMAB438Ra24 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| USEB438Ra | 酪氨酸羥化酶(TH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB438Ra | 酪氨酸羥化酶(TH)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB438Ra | 酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB438Ra01 | 酪氨酸羥化酶(TH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| PLOS ONE | Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome Sequencing [Pubmed:25181484] |
| biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
| Therapeutics?and?Clinical?Risk?Management | Elevated?blood?plasma?levels?of?epinephrine,?norepinephrine,?tyrosine?hydroxylase, TGFβ1, and TNFα?associated?with?high-altitude?pulmonary?edema?in an Indian population. [pubmed:27540296] |
| Sci Rep.? | Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. [pubmed:28230187] |
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| Environmental?Science?& Technology | Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonate [Pubmed:29565584] |
| Molecular Neurobiology | Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD Model [Pubmed:29429051] |
| Chemosphere | Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvae [Pubmed:29407805] |
| Neurospine | The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem Cells [Pubmed:29656620] |
| Toxicology and?Applied?Pharmacology | Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effects [Pubmed: 30222980] |
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| Environment International | Perfluoropolyether carboxylic acids (novel alternatives to PFOA) impair zebrafish posterior swim bladder development via thyroid hormone disruption [Pubmed: 31733528] |
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| International Journal of Pharmacology | Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease [Pubmed: 32084576] |
| comparative biochemistry and physiology c-toxicology & pharmacology | Thyroid disruption and developmental toxicity caused by Cd2+ in Schizopygopsis younghusbandi larvae [Pubmed: 32344129] |
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