酪氨酸羥化酶(TH)檢測試劑盒(化學發(fā)光免疫分析法)
CLIA Kit for Tyrosine Hydroxylase (TH)
TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14
- 編號USCB438Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長2h, 40min
- 檢測范圍13.7-10,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.6pg/mL.
- 樣本類型Serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1915 ¥ 2736 ¥ 12312 ¥ 23256 ¥ 191520
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測酪氨酸羥化酶(TH)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的酪氨酸羥化酶(TH)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-98 | 90 |
| EDTA plasma(n=5) | 87-105 | 101 |
| heparin plasma(n=5) | 90-97 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的酪氨酸羥化酶(TH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中酪氨酸羥化酶(TH)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-104% | 96-104% | 95-103% | 85-98% |
| EDTA plasma(n=5) | 89-96% | 78-88% | 80-94% | 99-105% |
| heparin plasma(n=5) | 98-105% | 88-95% | 82-92% | 92-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分鐘；
8. 讀數(shù)。
實驗原理
將酪氨酸羥化酶(TH)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的酪氨酸羥化酶(TH)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的酪氨酸羥化酶(TH)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的酪氨酸羥化酶(TH)呈正相關(guān)。用化學發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPB438Ra01 | 酪氨酸羥化酶(TH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB438Ra01 | 酪氨酸羥化酶(TH)多克隆抗體 | WB; IHC |
| ULAB438Ra71 | 酪氨酸羥化酶(TH)多克隆抗體(生物素標記) | WB |
| UMAB438Ra25 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| UMAB438Ra21 | 酪氨酸羥化酶(TH)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAB438Ra22 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| UMAB438Ra27 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| UMAB438Ra23 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| UMAB438Ra24 | 酪氨酸羥化酶(TH)單克隆抗體 | WB |
| USEB438Ra | 酪氨酸羥化酶(TH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB438Ra | 酪氨酸羥化酶(TH)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB438Ra | 酪氨酸羥化酶(TH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB438Ra01 | 酪氨酸羥化酶(TH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Mol Neurobiol. | 1, 25-dyhydroxyvitamin D3 Attenuates l-DOPA-Induced Neurotoxicity in Neural Stem Cells [Pubmed:25102940] |
| PLOS ONE | Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome Sequencing [Pubmed:25181484] |
| biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
| Therapeutics?and?Clinical?Risk?Management | Elevated?blood?plasma?levels?of?epinephrine,?norepinephrine,?tyrosine?hydroxylase, TGFβ1, and TNFα?associated?with?high-altitude?pulmonary?edema?in an Indian population. [pubmed:27540296] |
| Sci Rep.? | Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. [pubmed:28230187] |
| Journal of Cellular Biochemistry | Vaccarin administration ameliorates hypertension and cardiovascular remodeling in renovascular hypertensive rats [pubmed:28681939] |
| Environmental?Science?& Technology | Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonate [Pubmed:29565584] |
| Molecular Neurobiology | Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD Model [Pubmed:29429051] |
| Chemosphere | Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvae [Pubmed:29407805] |
| Neurospine | The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem Cells [Pubmed:29656620] |
| Toxicology and?Applied?Pharmacology | Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effects [Pubmed: 30222980] |
| International Journal Of Cardiology | Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus [Pubmed: 30581101] |
| Behavioural?Brain?Research | Hydroxychloroquine antiparkinsonian potential: Nurr1 modulation versus autophagy inhibition [] |
| Environment International | Perfluoropolyether carboxylic acids (novel alternatives to PFOA) impair zebrafish posterior swim bladder development via thyroid hormone disruption [Pubmed: 31733528] |
| Environmental Pollution | Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae [Pubmed: 31935612] |
| International Journal of Pharmacology | Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease [Pubmed: 32084576] |
| comparative biochemistry and physiology c-toxicology & pharmacology | Thyroid disruption and developmental toxicity caused by Cd2+ in Schizopygopsis younghusbandi larvae [Pubmed: 32344129] |
| International Journal of Stem cell Research & Therapy | Selection of Cells for Parkinson's Disease Cell-Therapy [] |
| Ecotoxicol Environ Saf | Effects of SiO2 nanoparticles on the uptake of tetrabromobisphenol A and its impact on the thyroid endocrine system in zebrafish larvae [33385677] |
| Nutr Res | Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and?… [Pubmed:35065349] |
| Food Chem Toxicol | Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulation [Pubmed:35439590] |
| Food Chemistry: Molecular Sciences | Unravelling the neuroprotective mechanisms of carotenes in differentiated human neural cells: biochemical and proteomic approaches [Pubmed:35415676] |
| Journal of Environmental Sciences | Parental and transgenerational impairments of thyroid endocrine system in zebrafish by 2, 4, 6-tribromophenol [] |
