胃蛋白酶原A(PGA)單克隆抗體
Monoclonal Antibody to Pepsinogen A (PGA)
PGA5; PG-A; Pepsinogen 5 Group I; Pepsinogen-5; Pepsin A-5
- 編號UMAA165Hu26
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C11
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPA165Hu01-胃蛋白酶原A(PGA)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 363 ¥ 846 ¥ 1209 ¥ 3023 ¥ 12090
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P;
Sample: Porcine Stomach Tissue
Primary Ab: 30μg/ml Mouse Anti-Human PGA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
AF488 staining on IF;
Sample: HEL92.1.7 cell
Primary Ab: 20μg/ml Mouse Anti-Human PGA Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Samples: Lane1: Human Urine; Lane2: Porcine Stomach lysate; Primary Ab: 0.2μg/ml Mouse Anti-Human PGA Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Samples: Lane1: Porcine Stomach lysate; Lane2: Rat Stomach lysate; Lane3: Canine Stomach lysate;
Primary Ab: 0.1μg/ml Mouse Anti-Human PGA Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Human THP1 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10μg/ml mouse Anti-human PGA Monoclonal Antibody (Catalog MAA165Hu26, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對PGA的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別PGA。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA165Hu01 | 胃蛋白酶原A(PGA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA165Hu01 | 胃蛋白酶原A(PGA)多克隆抗體 | WB; IHC; FCM |
| ULAA165Hu71 | 胃蛋白酶原A(PGA)多克隆抗體(生物素標記) | IHC |
| UMAA165Hu21 | 胃蛋白酶原A(PGA)單克隆抗體 | WB; IHC |
| UMAA165Hu28 | 胃蛋白酶原A(PGA)單克隆抗體 | WB; IHC |
| UMAA165Hu24 | 胃蛋白酶原A(PGA)單克隆抗體 | WB; IHC |
| UMAA165Hu25 | 胃蛋白酶原A(PGA)單克隆抗體 | WB; IHC; FCM |
| UMAA165Hu27 | 胃蛋白酶原A(PGA)單克隆抗體 | WB; IHC |
| UMAA165Hu29 | 胃蛋白酶原A(PGA)單克隆抗體 | WB |
| UMAA165Hu22 | 胃蛋白酶原A(PGA)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA165Hu26 | 胃蛋白酶原A(PGA)單克隆抗體 | WB; IHC; ICC/IF; FCM |
| UMAA165Hu23 | 胃蛋白酶原A(PGA)單克隆抗體 | WB; IHC |
| UFAA165Hu08 | 抗胃蛋白酶原A(PGA)單克隆抗體 | FCM |
| UFAA165Hu02 | 抗胃蛋白酶原A(PGA)單克隆抗體 | FCM |
| UFAA165Hu06 | 抗胃蛋白酶原A(PGA)單克隆抗體 | FCM |
| UFAA165Hu04 | 抗胃蛋白酶原A(PGA)單克隆抗體 | FCM |
| ULAA165Hu72 | 胃蛋白酶原A(PGA)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA165Hu | 胃蛋白酶原A(PGA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA165Hu | 胃蛋白酶原A(PGA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA165Hu01 | 胃蛋白酶原A(PGA)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA165Hu01 | 胃蛋白酶原A(PGA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| the journal of international advanced otology | Role of Laryngopharyngeal Reflux in the Pathogenesis of Otitis Media with Effusion [PubMed: 26223722] |
| Sensors (Basel) | Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed Electrode [Pubmed:29882917] |
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