白介素18(IL18)單克隆抗體
Monoclonal Antibody to Interleukin 18 (IL18)
IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma
- 編號(hào)UMAA064Hu22
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C10
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPA064Hu02-白介素18(IL18)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 275 ¥ 641 ¥ 915 ¥ 2288 ¥ 9150
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Spleen Tissue; Primary Ab: 20μg/mL Mouse Anti-Human IL18 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20μg/ml Mouse Anti-Human IL18 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Human IL18 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Spleen Tissue
Primary Ab: 20μg/ml Mouse Anti-Human IL18 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Figure. Western Blot; Sample: Recombinant IL18, Human.
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Western Blot; Sample: Lane1: HepG2 cell lysate; Lane2: Hela cell lysate; Lane3: Porcine Spleen lysate; Lane4: Rat Spleen lysate<br/>Primary Ab: 2μg/mL Mouse Anti-Human IL18 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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Western Blot; Sample: THP1 cell lysate
Primary Ab: 0.2μg/ml Mouse Anti-Human IL18 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: HaCaT cell lysate
Primary Ab: 0.2μg/ml Mouse Anti-Human IL18 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)IL18的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別IL18。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA064Hu01 | 白介素18(IL18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA064Hu01 | 白介素18(IL18)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA064Hu02 | 白介素18(IL18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA064Hu61 | 白介素18(IL18)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA064Hu01 | 白介素18(IL18)多克隆抗體 | WB; IHC |
| ULAA064Hu71 | 白介素18(IL18)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA064Hu81 | 白介素18(IL18)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| URAA064Hu21 | 白介素18(IL18)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA064Hu22 | 白介素18(IL18)單克隆抗體 | WB; IHC |
| UMAA064Hu23 | 白介素18(IL18)單克隆抗體 | WB; IHC |
| UMAA064Hu25 | 白介素18(IL18)單克隆抗體 | IHC |
| UMAA064Hu27 | 白介素18(IL18)單克隆抗體 | WB |
| UMAA064Hu21 | 白介素18(IL18)單克隆抗體 | WB; IHC |
| UMAA064Hu29 | 白介素18(IL18)單克隆抗體 | WB; IHC |
| UMAA064Hu24 | 白介素18(IL18)單克隆抗體 | IHC |
| UMAA064Hu28 | 白介素18(IL18)單克隆抗體 | WB |
| UMAA064Hu26 | 白介素18(IL18)單克隆抗體 | WB |
| UFAA064Hu02 | 抗白介素18(IL18)單克隆抗體 | FCM |
| UFAA064Hu08 | 抗白介素18(IL18)單克隆抗體 | FCM |
| UFAA064Hu04 | 抗白介素18(IL18)單克隆抗體 | FCM |
| UFAA064Hu06 | 抗白介素18(IL18)單克隆抗體 | FCM |
| USEA064Hu | 白介素18(IL18)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA064Hu | 白介素18(IL18)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA064Hu | 白介素18(IL18)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA064Hu | 白介素18(IL18)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA064Hu | 白介素18(IL18)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA064Hu01 | 白介素18(IL18)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
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| Journal of?Neuroinflammation | Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes [Pubmed:29357878] |
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| Chemico-Biological?Interactions | Nifuroxazide, a STAT3 inhibitor, mitigates inflammatory burden and protects against diabetes-induced nephropathy in rats [Pubmed:29291386] |
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| International Journal of Molecular?Medicine | MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing?… [Pubmed:29512778] |
| Archives of Virology | Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China [Pubmed:29305646] |
| Journal of Inflammation | Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus [Pubmed: 30202244] |
| Journal of Affective Disorders | Post-stroke depression and cognitive impairment: study design and preliminary findings in a Brazilian prospective stroke cohort (EMMA study) [Doi: 10.1016/j.jad.2018.10.003] |
| BioScientia Medicina | Sensitivity and Spesificity of Interleukin 18 as Biomarker for Acute Kidney Injury [] |
| International Journal of Molecular Medicine | Ginsenoside Rg1 protects against H2O2?induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in vitro [Pubmed: 30535505] |
| Nutrients | Effect of Rosmarinic Acid on the Serum Parameters of Glucose and Lipid Metabolism and Oxidative Stress in Estrogen-Deficient Rats [Pubmed: 30691017] |
| International immunopharmacology | NADPH oxidase 2-mediated NLRP1 inflammasome activation involves in neuronal senescence in hippocampal neurons in vitro [Pubmed: 30677569] |
| Acta?Histochemica | Pre-administration of luteoline attenuates neonatal sevoflurane-induced neurotoxicity in mice [Doi: 10.1016/j.acthis.2019.04.004] |
| JOURNAL OF DRUG TARGETING | Artemisinin alleviates atherosclerotic lesion by reducing macrophage inflammation via regulation of AMPK/NF-κB/NLRP3 inflammasomes pathway [Pubmed: 31094238] |
| IUBMB Life | Melatonin Enhances Autophagy and Decreases Apoptosis Induced by nanosilica in RAW264. 7 cells [Pubmed: 31018046] |
| American Journal of Reproductive Immunology | Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women [Pubmed: 31148259] |
| INFLAMMATION RESEARCH | TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis [Pubmed: 31119302] |
| PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model [Pubmed: 31247004] |
| Journal of Dairy Science | Hepatic nuclear factor kappa B signaling pathway and NLR family pyrin domain containing 3 inflammasome is over-activated in ketotic dairy cows [Pubmed: 31495623] |
| Rheumatology international | Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in?… [Pubmed: 31468124] |
| LIFE SCIENCES | Protective effects of epigallocatechin gallate against ischemia reperfusion injury in rat skeletal muscle via activating Nrf2/HO-1 signaling pathway [Pubmed: 31678278] |
| life sciences | Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome [Pubmed: 31738882] |
| International Immunopharmacology | Dexamethasone alleviate allergic airway inflammation in mice by inhibiting the activation of NLRP3 inflammasome [Pubmed: 31780368] |
| Journal of Food Science | Effects of Total Flavonoids from Exocarpium Citri Grandis on Air Pollution Particle‐Induced Pulmonary Inflammation and Oxidative Stress in Mice [Pubmed: 31762039] |
| Antioxidants | Effect of Rosmarinic Acid and Sinapic Acid on Oxidative Stress Parameters in the Cardiac Tissue and Serum of Type 2 Diabetic Female Rats [Pubmed: 31771099] |
| Frontiers in Immunology | The Increase in IL-1β in the Early Stage of Heatstroke Might Be Caused by Splenic Lymphocyte Pyroptosis Induced by mtROS-Mediated Activation of the?… [Pubmed: 31921131] |
| Pharmacological Reports | The dual reno-and neuro-protective effects of dimethyl fumarate against uremic encephalopathy in a renal ischemia/reperfusion model [Pubmed: 32141026] |
| Experimental and Therapeutic Medicine | Induction of autophagy protects human dental pulp cells from lipopolysaccharide?induced pyroptotic cell death [Pubmed: 32104285] |
| Cancer Chemother Pharmacol | Novel complementary antitumour effects of celastrol and metformin by targeting IκBκB, apoptosis and NLRP3 inflammasome activation in diethylnitrosamine-induced … [Pubmed: 31989218] |
| Frontiers in Immunology | TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation [Pubmed: 32117301] |
| molecular immunology | STAT5a induces endotoxin tolerance by alleviating pyroptosis in kupffer cells [Pubmed: 32298872] |
| INVESTIGATIONAL NEW DRUGS | Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors [Pubmed: 32772342] |
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| Inflammopharmacology | Novel complementary coloprotective effects of metformin and MCC950 by modulating HSP90/NLRP3 interaction and inducing autophagy in rats [Pubmed: 32594364] |
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| Molecular Medicine Reports | Effects of PYRIN-containing Apaf1-like protein 1 on isoflurane-induced postoperative cognitive dysfunction in aged rats [Pubmed: 32626997] |
| Food and Agricultural Immunology | Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells [] |
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| Biomarkers of Allergic Asthma and their Association with Serum Parameters [] | |
| Drug Dev Res | Paeoniflorin inhibited nod?\like receptor protein?\3 inflammasome and NF?\|êB?\mediated inflammatory reactions in diabetic foot ulcer by inhibiting the chemokine??- [33236457] |
| Molecules | Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion [33430011] |
| Mol Cell Biochem | Intermittent high glucose induces pyroptosis of rat H9C2 cardiomyocytes via sodium¨Cglucose cotransporter 1 [33608832] |
| Life Sci | Inhibition of SGLT1 protects against glycemic variability-induced cardiac damage and pyroptosis of cardiomyocytes in diabetic mice [33508297] |
| Tissue Cell | Protective effects of Oridonin against cerebral ischemia/reperfusion injury by inhibiting the NLRP3 inflammasome activation [33676236] |
| Inflammopharmacology | Dapagliflozin, an SGLT2 inhibitor, ameliorates acetic acid-induced colitis in rats by targeting NF|êB/AMPK/NLRP3 axis [34002329] |
| Arch Biochem Biophys | Vitamin D ameliorates high-fat-diet-induced hepatic injury via inhibiting pyroptosis and alters gut microbiota in rats [33965368] |
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| J Mol Cell Cardiol | CircHelz activates NLRP3 inflammasome to promote myocardial injury by sponging miR-133a-3p in mouse ischemic heart [34043986] |
| Med Sci Monit | Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating ?- [34059615] |
| Biological Chemistry | HDAC8 promotes daunorubicin resistance of human acute myeloid leukemia cells via regulation of IL-6 and IL-8 [33938176] |
| BioMed Research International | Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves??- [33376718] |
| Evidence-Based Complementary and Alternative Medicine | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling??- [33505502] |
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| J Neuroinflammation | Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia–reperfusion injury via astrocyte NF-κB/NLRP3 inflammasome pathway [34362408] |
| Mol Med Rep | Sacubitril/valsartan (LCZ696) reduces myocardial injury following myocardial infarction by inhibiting NLRP3?induced pyroptosis via the TAK1/JNK signaling pathway [34296299] |
| Int Immunopharmacol | The protective effect of isoflurane pretreatment on liver IRI by suppressing noncanonical pyroptosis of liver macrophages [34332342] |
| LIFE SCIENCES | The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation [34560082] |
| Biochim Biophys Acta Mol Cell Biol Lipids | Elaidic acid induced NLRP3 inflammasome activation via ERS-MAPK signaling pathways in Kupffer cells [34610469] |
| Inflammation | Emodin Attenuates LPS-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome-Dependent Pyroptosis Signaling Pathway In vitro and In vivo [34787801] |
| J Agric Food Chem | Allicin Ameliorates Intestinal Barrier Damage via Microbiota-Regulated Short-Chain Fatty Acids-TLR4/MyD88/NF-κB Cascade Response in Acrylamide-Induced Rats [34694121] |
| Journal of Inflammation Research | The Supportive Role of NSC328382, a P2X7R Antagonist, in Enhancing the Inhibitory Effect of CRID3 on NLRP3 Inflammasome Activation in Rats with Dextran?… [34321905] |
| Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice [34960627] |
| Toxicology | Salidroside alleviates liver inflammation in furan-induced mice by regulating oxidative stress and endoplasmic reticulum stress [34450210] |
| Antioxidants | Key Mechanisms and Potential Implications of Hericium erinaceus in NLRP3 Inflammasome Activation by Reactive Oxygen Species during Alzheimer's Disease [34829535] |
| Ecotoxicol Environ Saf | Silica nanoparticles induce pulmonary autophagy dysfunction and epithelial-to-mesenchymal transition via p62/NF-κB signaling pathway [Pubmed:35158278] |
| J Dairy Sci | Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB?… [Pubmed:35248377] |
| Free Radic Biol Med | Silica nanoparticles induce pyroptosis and cardiac hypertrophy via ROS/NLRP3/Caspase-1 pathway [Pubmed:35219847] |
| International Heart Journal | Melatonin Inhibits OGD/R-Induced H9c2 Cardiomyocyte Pyroptosis via Regulation of MT2/miR-155/FOXO3a/ARC Axis [Pubmed:35354753] |
| Journal of Integrative Neuroscience | Gastrodin suppresses pyroptosis and exerts neuroprotective effect in traumatic brain injury model by inhibiting NLRP3 inflammasome signaling pathway [Pubmed:35364660] |
| Perfusion | Insulin reduces pyroptosis-induced inflammation by PDHA1 dephosphorylation-mediated NLRP3 activation during myocardial ischemia-reperfusion injury [Pubmed:35506656] |
| Ecotoxicol Environ Saf | Ambient ozone exposure induces ROS related-mitophagy and pyroptosis via NLRP3 inflammasome activation in rat lung cells [Pubmed:35642860] |
| Medicina | Plasma Concentrations of Cytokines in Patients with Combined Hyperlipidemia and Atherosclerotic Plaque before Treatment Initiation—A Pilot Study [Pubmed:35630041] |
| Pyroptosis Accelerates Spiral Ganglion Neuronal Degeneration Induced by Aminoglycosides in the Cochlea [] | |
| researchsquare | Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling [] |
| Reseach square | Assessment of selected interleukins (IL-6, IL-17A, IL-18, IL-23) and chemokines (RANTES, IP-10) in children with acute and chronic urticaria [] |
| Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS [] |
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