低氧誘導因子1α(HIF1a)單克隆抗體
Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
- 編號UMAA798Hu27
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)D2
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPA798Hu02-低氧誘導因子1α(HIF1a)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應用IHC; ICC/IF
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 338 ¥ 788 ¥ 1125 ¥ 2813 ¥ 11250
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Porcine Cardiac Muscle Tissue Primary Ab: 20μg/ml Mouse Anti-Human HIF1a Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Human HIF1a Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue
Primary Ab: 40μg/ml Mouse Anti-Human HIF1a Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 40μg/ml Mouse Anti-Human HIF1a Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 30μg/ml Mouse Anti-Human HIF1a Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對HIF1a的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別HIF1a。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UAPA798Hu02 | 低氧誘導因子1α(HIF1a)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA798Hu01 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA798Hu02 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA798Hu01 | 低氧誘導因子1α(HIF1a)多克隆抗體 | WB; IHC |
| UPAA798Hu02 | 低氧誘導因子1α(HIF1a)多克隆抗體 | WB; IHC |
| ULAA798Hu81 | 低氧誘導因子1α(HIF1a)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA798Hu71 | 低氧誘導因子1α(HIF1a)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA798Hu21 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA798Hu23 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; ICC/IF |
| UMAA798Hu24 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB |
| UMAA798Hu25 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB |
| UMAA798Hu22 | 低氧誘導因子1α(HIF1a)單克隆抗體 | WB; IHC |
| UMAA798Hu27 | 低氧誘導因子1α(HIF1a)單克隆抗體 | IHC; ICC/IF |
| UMAA798Hu29 | 低氧誘導因子1α(HIF1a)單克隆抗體 | IHC; ICC/IF |
| USEA798Hu | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA798Hu | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA798Hu | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA798Hu | 低氧誘導因子1α(HIF1a)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA798Hu | 低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA798Hu01 | 低氧誘導因子1α(HIF1a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Journal of Physiological Anthropology | Telomeres are elongated in rats exposed to moderate altitude [Pubmed:Pmc4088304] |
| Molecular Medicine Reports | Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. [Pubmed:25384583] |
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| Bulletin of Experimental Biology and Medicine | Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar Rats [Pubmed: 30903510] |
| The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency [] | |
| scandinavian journal of immunology | Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathway [Pubmed: 31055848] |
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| Tissue Eng Part A | Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation [Pubmed: 31238789] |
| ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ [] | |
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| Stem Cell Res Ther | Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus [Pubmed: 32894200] |
| J Cell Physiol | PLCε regulates metabolism and metastasis signaling via HIF‐1α/MEK/ERK pathway in prostate cancer [Pubmed: 32383180] |
| Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза [] | |
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| EXPERIMENTAL EYE RESEARCH | Astaxanthin mediated regulation of VEGF through HIF1|á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model [33789142] |
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| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
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