雙調(diào)蛋白(AREG)單克隆抗體
Monoclonal Antibody to Amphiregulin (AREG)
AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor
- 編號(hào)UMAA006Hu24
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))A3
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA006Hu01-雙調(diào)蛋白(AREG)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 270 ¥ 630 ¥ 900 ¥ 2250 ¥ 9000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 30μg/ml Mouse Anti-Human AREG Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 30μg/ml Mouse Anti-Human AREG Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 20μg/ml Mouse Anti-Human AREG Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11) -
AF488 staining on IF;<br/>Sample: Hela cell<br/>Primary Ab: 30μg/ml Mouse Anti-Human AREG Antibody<br/>Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu11)
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Figure. Western Blot; Sample: Recombinant AREG, Human.
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Western Blot; Sample: Rat Placenta lysate;
Primary Ab: 3μg/ml Mouse Anti-Human AREG Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Human MCF7 cell lysate; <br/>Primary Ab: 3μg/ml Mouse Anti-Human AREG Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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Western Blot; Sample: Rat Cerebrum lysate<br/>Primary Ab: 0.1μg/ml Mouse Anti-Human AREG Antibody<br/>Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對AREG的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別AREG。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunofluorescence: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA006Hu01 | 雙調(diào)蛋白(AREG)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA006Hu01 | 雙調(diào)蛋白(AREG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA006Hu01 | 雙調(diào)蛋白(AREG)多克隆抗體 | WB; IHC |
| UMAA006Hu23 | 雙調(diào)蛋白(AREG)單克隆抗體 | IHC; ICC/IF |
| UMAA006Hu25 | 雙調(diào)蛋白(AREG)單克隆抗體 | IHC |
| UMAA006Hu22 | 雙調(diào)蛋白(AREG)單克隆抗體 | IHC |
| UMAA006Hu24 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA006Hu26 | 雙調(diào)蛋白(AREG)單克隆抗體 | WB; IHC |
| UMAA006Hu21 | 雙調(diào)蛋白(AREG)單克隆抗體 | IHC |
| UMAA006Hu27 | 雙調(diào)蛋白(AREG)單克隆抗體 | ICC/IF |
| UMAA006Hu29 | 雙調(diào)蛋白(AREG)單克隆抗體 | ICC/IF |
| ULAA006Hu76 | 雙調(diào)蛋白(AREG)單克隆抗體(生物素標(biāo)記) | IHC |
| USEA006Hu | 雙調(diào)蛋白(AREG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA006Hu | 雙調(diào)蛋白(AREG)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA006Hu | 雙調(diào)蛋白(AREG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA006Hu01 | 雙調(diào)蛋白(AREG)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA006Hu01 | 雙調(diào)蛋白(AREG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Onkologie | Amphiregulin and Epiregulin Expression in Colorectal Carcinoma and the Correlation with Clinicopathological Characteristics [Karger: 315380] |
| Br J Cancer. | Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer. [Pubmed:24800946] |
| Cancer Sci | Association between serum ligands and the skin toxicity of anti-epidermal growth factor receptor antibody in metastatic colorectal cancer [PubMed: 25707609] |
| J Cancer Res Clin Oncol | Amphiregulin confers trastuzumab resistance via AKT and ERK activation in HER2-positive breast cancer [PubMed: 26195282] |
| Oncotarget.? | Serum?level?of?hepatocyte?growth?factor?is a?novel?marker?of?predicting?theoutcome?and?resistance?to the?treatment?with?trastuzumab?in HER2-positive patients with metastatic gastric cancer [Pubmed:26716644] |
| Acta?Pharmacologica Sinica | Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi') [Pubmed: 19363516] |
| International Journal of Biological Sciences | CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17 [] |
| Neoplasia | Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets [Pubmed: 33164313] |
