皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Cortisol (Cor) ,etc. by FLIA (Flow Luminescence Immunoassay)
Hydrocortisone
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA462Rb
- 物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長1.5h
- 檢測范圍0.98-1000ng/mL
- 靈敏度最小可檢測劑量小于等于0.327 ng/mL.
- 樣本類型Serum, plasma, urine and other biological fluids
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-103 | 90 |
| EDTA plasma(n=5) | 79-95 | 82 |
| heparin plasma(n=5) | 80-103 | 92 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-101% | 95-103% | 80-97% | 80-89% |
| EDTA plasma(n=5) | 94-102% | 97-105% | 80-96% | 80-105% |
| heparin plasma(n=5) | 79-94% | 87-95% | 91-98% | 86-96% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCEA462Rb | 皮質(zhì)醇(Cor)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA462Rb | 皮質(zhì)醇(Cor)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Research in Veterinary Science | Use of infrared thermography to assess the influence of high environmental temperature on rabbits [ScienceDirect: S0034528813001380] |
| British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human b-defensin-1 and human b-defensin-2 in the human milk of Han Chinese [Pubmed:24124699] |
| Theriogenology | Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit model [Pubmed:25442388] |
| Animal Production Science | Effect of high temperatures on breeding rabbit behaviour [Publish:Source] |
| JBCD | Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental students [Index.Php: Jbcd] |
| International Journal of Immunopathology & Pharmacology | Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638] |
| IET Micro & Nano Letters | Surface plasmon resonance immunoassay for cortisol determination with a self-assembling denaturalised bovine serum albumin layer on surface plasmon resonance chip [xpls:7393900] |
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| Journal?of?Dairy?Science | Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves [publication:306322503] |
| Acta Veterinaria Hungarica | Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation [acve-2017-0006] |
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| MICROBIAL PATHOGENESIS | Assessment of certain biomarkers for predicting survival in response to treatment in dogs naturally infected with canine parvovirus [Pubmed: 32926999] |
| AQUACULTURE RESEARCH | Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition [] |
| AQUACULTURE RESEARCH | Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense [] |
| Anti-stress activity of some plants extracts of the North Caucasus flora [] | |
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| J Am Heart Assoc | Post?\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest [33960200] |
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