低氧誘導因子1α(HIF1a)重組蛋白
Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
- 編號URPA798Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞核, 細胞質(zhì)
- 預測分子量28.3kDa
- 實際分子量33kDa(差異分析請參閱說明書)
- 片段與標簽Arg588~Asn836 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點6.3
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 420 ¥ 1050 ¥ 2100 ¥ 6300 ¥ 15750
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA798Mu01 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA798Mu02 | 低氧誘導因子1α(HIF1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA798Mu01 | 低氧誘導因子1α(HIF1a)多克隆抗體 | WB |
| UPAA798Mu02 | 低氧誘導因子1α(HIF1a)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA798Mu81 | 低氧誘導因子1α(HIF1a)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA798Mu71 | 低氧誘導因子1α(HIF1a)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA798Mu | 低氧誘導因子1α(HIF1a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA798Mu | 低氧誘導因子1α(HIF1a)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA798Mu | 低氧誘導因子1α(HIF1a)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA798Mu01 | 低氧誘導因子1α(HIF1a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Archives of Gynecology and Obstetrics | Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? [10.1007/s00404-017-4461-2] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee [pubmed:28123469] |
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| Microchimica?Acta | Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric test based on click-mediated growth of gold nanoparticles [Pubmed: 30209641] |
| Bulletin?of Experimental?Biology?and?Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in?… [Pubmed: 30353332] |
| Translational?Oncology | OKN-007 Increases temozolomide (TMZ) Sensitivity and Suppresses TMZ-Resistant Glioblastoma (GBM) Tumor Growth [Pubmed: 30468988] |
| West Indian Medical Journal | Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia. [] |
| Bulletin of Experimental Biology and Medicine | Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar Rats [Pubmed: 30903510] |
| The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency [] | |
| scandinavian journal of immunology | Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathway [Pubmed: 31055848] |
| JOURNAL OF PERIODONTOLOGY | A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A?… [Pubmed: 31020669] |
| Tissue Eng Part A | Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder Augmentation [Pubmed: 31238789] |
| ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ [] | |
| molecular and cellular biochemistry | Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia [Pubmed: 31420792] |
| Molecules | Regulation of MicroRNA-155 and Its Related Genes Expression by Inositol Hexaphosphate in Colon Cancer Cells [Pubmed: 31744065] |
| Stem Cell Res Ther | Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus [Pubmed: 32894200] |
| J Cell Physiol | PLCε regulates metabolism and metastasis signaling via HIF‐1α/MEK/ERK pathway in prostate cancer [Pubmed: 32383180] |
| Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза [] | |
| Food Funct | Prenatal vitamin D supplementation reduces blood pressure and improves placental angiogenesis in an animal model of preeclampsia [33237074] |
| Eur J Pharmacol | Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ?- [33705802] |
| EXPERIMENTAL EYE RESEARCH | Astaxanthin mediated regulation of VEGF through HIF1|á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model [33789142] |
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