血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Serum Amyloid A (SAA)
SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測血清淀粉樣蛋白A(SAA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的血清淀粉樣蛋白A(SAA)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-94 | 91 |
| EDTA plasma(n=5) | 80-99 | 87 |
| heparin plasma(n=5) | 80-96 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血清淀粉樣蛋白A(SAA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血清淀粉樣蛋白A(SAA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-105% | 78-104% | 98-105% | 83-90% |
| EDTA plasma(n=5) | 94-104% | 87-95% | 78-90% | 81-99% |
| heparin plasma(n=5) | 85-95% | 79-90% | 99-105% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將血清淀粉樣蛋白A(SAA)單克隆抗體包被微孔板，制成固相載 體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA885Mu04 | 血清淀粉樣蛋白A(SAA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA885Mu02 | 血清淀粉樣蛋白A(SAA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA885Mu01 | 血清淀粉樣蛋白A(SAA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA885Mu03 | 血清淀粉樣蛋白A(SAA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA885Mu02 | 血清淀粉樣蛋白A(SAA)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA885Mu05 | 血清淀粉樣蛋白A(SAA)多克隆抗體 | WB; IHC |
| UPAA885Mu03 | 血清淀粉樣蛋白A(SAA)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA885Mu01 | 血清淀粉樣蛋白A(SAA)多克隆抗體 | IHC |
| ULAA885Mu41 | 血清淀粉樣蛋白A(SAA)多克隆抗體(藻紅素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA885Mu81 | 血清淀粉樣蛋白A(SAA)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA885Mu71 | 血清淀粉樣蛋白A(SAA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA885Mu | 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA885Mu | 血清淀粉樣蛋白A(SAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA885Mu | 血清淀粉樣蛋白A(SAA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA885Mu01 | 血清淀粉樣蛋白A(SAA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Nitric Oxide. | Effect of feeding a high fat diet on hydrogen sulfide (H 2 S) metabolism in the mouse [Pubmed:24637018] |
| Open Biochem J. | Acute Phase Response: Implication in ST-segment Elevation Myocardial Infarction [Pubmed:24894970] |
| Iran J Pharm Res. | Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical Trial [Pubmed:25237354] |
| US National Library of Medicine National Institutes of Health | No association between vitamin D levels and inflammation markers in patients with acute coronary syndrome [Pubmed:25615956] |
| BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
| BOSCA | EFFECT OF UTERINE IMMUNOMODULATION ON SERUM AMYLOID-A CONCENTRATION AND CONCEPTION RATE IN CYCLIC NON BREEDING COWS [Tcrjournals:Source] |
| Life Sciences | Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew diet [PubMed: 26188592] |
| Endocrine | Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitus [PubMed: 26049370] |
| J Assist Reprod Genet. | The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndrome [PubMed: 26463878] |
| International Journal of Clinical and Experimental Medicine | Circulating serum amyloid A: a potential biomarker for infection after aneurysmal subarachnoid hemorrhage [files:ijcem0021201] |
| PLoS One. | An Anti-β-Amyloid Vaccine for Treating Cognitive Deficits in a Mouse Model of Down Syndrome [Pubmed:27023444] |
| United States Patent Application | PATHWAY SPECIFIC MARKERS FOR DIAGNOSING IRRITABLE BOWEL SYNDROME [y2016:0139148.html] |
| Veterinary World | Acute phase response in lame crossbred dairy cattle [pubmed:27956769] |
| Nature Communications | A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense [Pubmed:29410422] |
| Journal of?Animal?Sciences | Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations [Pubmed:30060086] |
| Journal?of?Animal?Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood?… [Pubmed: 30184125] |
| Journal of?Dairy?Science | Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows [Pubmed: 17430930] |
| European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341] |
| Research in Veterinary Science | Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows [] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
| PLoS One | Effects of vaccination on acute-phase protein response in broiler chicken [Pubmed: 32191767] |
| VETERINARY PATHOLOGY | AA Amyloid Deposition in the Central and Peripheral Nervous Systems in Flamingos [Pubmed: 32677873] |
| Comp Immunol Microbiol Infect Dis | Serum amyloid A as an marker of cow? s mastitis caused by Streptococcus sp. [Pubmed: 32505957] |
| Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy?… [] |
| РОЛЬ СЫВОРОТОЧНОГО АМИЛОИДА А И С-РЕАКТИВНОГО БЕЛКА В ПРОГНОЗИРОВАНИИ ПОСЛЕОПЕРАЦИОННЫХ ОСЛОЖНЕНИЙ И?… [] | |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient??- [33474564] |
| Animals | Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid [34070499] |
| peerj | Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats [33510972] |
| Arquivo Brasileiro de Medicina Veterin¨￠ria e Zootecnia | CCS e amiloide A do leite de quartos mam¨￠rios tratados na secagem com plasma rico em plaquetas aut¨?logo, associado ou n?o a antibi¨?tico [] |
| Probiotics Antimicrob Proteins | The Effect of Feed Supplementation with EM Bokashi? Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators?… [34596883] |
| Vet Anim Sci | Effects of dietary turmeric (Curcuma longa) on innate and acquired immune responses in broiler chicken [34765801] |
| Brain Sci | Mahanimbine Improved Aging-Related Memory Deficits in Mice through Enhanced Cholinergic Transmission and Suppressed Oxidative Stress, Amyloid Levels, and?… [Pubmed:35053756] |
| J Clin Med | A Three-Protein Panel to Support the Diagnosis of Sepsis in Children [Pubmed:35329889] |
| Diabetes Metabolic Syndrome and Obesity-Targets and Therapy | Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in India [Pubmed:35717896] |
| Clinical and Experimental Immunology | Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients [Pubmed:35348620] |
| ?neklerde Subklinik Mastitis Tan?s?nda Akut Faz Proteinlerinden Amiloid A ve C-Reaktif Protein Düzeylerinin De?erlendirilmesi [] |
