窖蛋白(CAV1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Caveolin 1 (CAV1)
VIP21; Caveolae Protein 22kDa
- 編號(hào)USEA214Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍0.24-15ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.09ng/mL.
- 樣本類型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1323 ¥ 1890 ¥ 8505 ¥ 16065 ¥ 132300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)窖蛋白(CAV1)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將窖蛋白(CAV1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的窖蛋白(CAV1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的窖蛋白(CAV1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的窖蛋白(CAV1)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA214Hu01 | 窖蛋白(CAV1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA214Hu21 | 窖蛋白(CAV1)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA214Hu08 | 窖蛋白(CAV1)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA214Hu01 | 窖蛋白(CAV1)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| UMAA214Hu22 | 窖蛋白(CAV1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA214Hu | 窖蛋白(CAV1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA214Hu | 窖蛋白(CAV1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA214Hu01 | 窖蛋白(CAV1)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Mendel Net | Novel prostate cancer tumour markers in a cell line model [Mendelu: source] |
| Oncology reports | Caveolin-1 as a potential high-risk prostate cancer biomarker [PubMed: 22159333] |
| Anticancer Research | Increased plasma caveolin-1 levels are associated with progression of prostate cancer among Japanese men [Pubmed: 23645736] |
| Sarcoidosis Vasc Diffuse Lung Dis.? | Decrased sputum caveolin-1 is associated with systemic sclerosis related lung disease [Pubmed:24751454] |
| Cancer Genomics and Proteomics | Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression [PubMed: 26543085] |
| Atherosclerosis | Genomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarction [Pubmed:26775120] |
| Arthritis Research & Therapy | Increased caveolin-1 in intervertebral disc degeneration facilitates repair [Pubmed:26939667] |
| Oxid Med Cell Longev | Lower Serum Caveolin-1 Is Associated with Cerebral Microbleeds in Patients with Acute Ischemic Stroke [Pubmed:27119011] |
| Inflammation | Caveolin-1?Promotes?the?Imbalance?of?Th17/Treg?in?Patients?with?Chronic?Obstructive?PulmonaryDisease. [pubmed:27613621] |
| Oxidative Medicine and Cellular Longevity | Swim Training Modulates Skeletal Muscle Energy Metabolism, Oxidative Stress, and Mitochondrial Cholesterol Content in Amyotrophic Lateral Sclerosis Mice [Pubmed:29849903] |
| Microcirculation | Endothelium‐dependent hyperpolarization‐mediated vasodilatation compensates nitric oxide‐mediated endothelial dysfunction during ischemia in diabetes‐induced?… [Pubmed:29665152] |
| Genetic Testing and Molecular Biomarkers | The CAV1 Gene 3′ Untranslated Region Single Nucleotide Polymorphisms Are Associated with the Risk of Pulmonary Hypertension in Chinese Han Chronic?… [] |
| Cell Rep | SIRT6 transcriptionally regulates fatty acid transport by suppressing PPAR|? [34077730] |
| researchsquare | Cav-1 Protein Levels in Serum and Infarcted Brain Correlate with Hemorrhagic Volume in a Mouse Model of Thromboembolic Stroke, Independently of Rt-PA??- [] |
| Molecules | 5, 2′-Dibromo-2, 4′, 5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein [Pubmed:35566232] |
