鐵調(diào)素(Hepc)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Hepcidin (Hepc)
HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor
- 編號(hào)USEB979Hu
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍62.5-4,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于26.4pg/mL.
- 樣本類(lèi)型serum, plasma and other biological fluids
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1722 ¥ 2460 ¥ 11070 ¥ 20910 ¥ 172200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)鐵調(diào)素(Hepc)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的鐵調(diào)素(Hepc)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| EDTA plasma(n=5) | 87-101 | 96 |
| heparin plasma(n=5) | 87-94 | 91 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的鐵調(diào)素(Hepc)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中鐵調(diào)素(Hepc)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 97-104% | 90-103% | 98-105% |
| EDTA plasma(n=5) | 86-101% | 83-92% | 84-105% | 96-103% |
| heparin plasma(n=5) | 84-103% | 86-94% | 80-95% | 87-97% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將鐵調(diào)素(Hepc)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的鐵調(diào)素(Hepc)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的鐵調(diào)素(Hepc)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的鐵調(diào)素(Hepc)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPB979Hu61 | 鐵調(diào)素(Hepc)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB979Hu01 | 鐵調(diào)素(Hepc)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB979Hu61 | 鐵調(diào)素(Hepc)活性蛋白 | Cell?culture;?Activity?Assays. |
| UCPB979Hu12 | 鐵調(diào)素(Hepc)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPB979Hu22 | 鐵調(diào)素(Hepc)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPB979Hu23 | 鐵調(diào)素(Hepc)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPB979Hu21 | 鐵調(diào)素(Hepc)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAB979Hu07 | 鐵調(diào)素(Hepc)多克隆抗體 | IHC |
| UPAB979Hu09 | 鐵調(diào)素(Hepc)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAB979Hu06 | 鐵調(diào)素(Hepc)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAB979Hu01 | 鐵調(diào)素(Hepc)多克隆抗體 | IHC |
| UPAB979Hu08 | 鐵調(diào)素(Hepc)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAB979Hu81 | 鐵調(diào)素(Hepc)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAB979Hu71 | 鐵調(diào)素(Hepc)多克隆抗體(生物素標(biāo)記) | IHC |
| UMAB979Hu26 | 鐵調(diào)素(Hepc)單克隆抗體 | Testing in progress |
| UMAB979Hu22 | 鐵調(diào)素(Hepc)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAB979Hu21 | 鐵調(diào)素(Hepc)單克隆抗體 | IHC |
| URAB979Hu21 | 鐵調(diào)素(Hepc)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| USEB979Hu | 鐵調(diào)素(Hepc)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEB979Hu | 鐵調(diào)素(Hepc)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB979Hu | 鐵調(diào)素(Hepc)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| UCCB979Hu | 鐵調(diào)素(Hepc)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB979Hu | 鐵調(diào)素(Hepc)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB979Hu11 | 鐵調(diào)素(Hepc)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Endocrine connections | Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression [pubmed:28179377] |
| Maternal and cord blood hepcidin levels based on gestational weeks in term and preterm infants [publication:305921433_Maternal_and_cord_blood_hepc] | |
| Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463] |
| The Royal Society of Chemistry | Silkworm feces extract improves iron deficiency anemia via suppressing hepcidin expression and promoting iron-regulatory proteins expression [DOI: 10.1039/C7RA09576G] |
| Journal of Inflammation | Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients [10.1186:s12950-017-0166-3] |
| Antioxidants and Redox Signaling | AMPK serves as a Therapeutic Target against Anemia of Inflammation [pubmed:27923278] |
| NutriciónHospitalaria | Micronutrient supplementation in gastric bypass surgery: prospective study on in??? ammation and iron metabolism in premenopausal women [10.20960/nh.1162] |
| The Japanese Society of Hematology | Hepcidin suppression in β?thalassemia is associated with the down?regulation of atonal homolog 8 [pubmed:28405918] |
| Tuberculosis | Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity [10.1016/j.tube.2017.11.011] |
| Oman Medical Journal | The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599] |
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| Cellular?Microbiology? | inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages [Pubmed:29470856] |
| Journal of Histochemistry & Cytochemistry | Acute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat Kidney [Pubmed:29873589] |
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| Hematology | Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? [Pubmed:29318945] |
| Hepcidin serum levels in HCV chronically mono-infected na?ve patients, compared to healthy individuals [77856] | |
| Food & Function | Effect of iron supplement from microalgae on the treatment of iron-deficient anemia in rats [Doi: 10.1039/C8FO01696H] |
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| Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats [] |
| Naunyn Schmiedebergs Arch Pharmacol. | 3-Amidinophenylalanine-derived matriptase inhibitors can modulate hepcidin production in vitro [Pubmed: 31659405] |
| DEVELOPMENT GROWTH & DIFFERENTIATION | H2O2 induces oxidative stress damage through the BMP‐6/SMAD/hepcidin axis [Pubmed: 32012242] |
| Frontiers in Physiology | Increased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior Rowers [Pubmed: 32038278] |
| J Korean Med Sci | Can Calprotectin Show Subclinical Inflammation in Familial Mediterranean Fever Patients? [Pubmed: 32174062] |
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| The Egyptian Journal of Haematology | Urinary hepcidin concentration in assessment of iron homeostasis in pediatrics [] |
| COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION [] | |
| Serum iron, prealbumin, D-dimer and prothrombin time are indicators of the diagnosis, severity and outcome of Salmonella enterica serovar Enteritidis infection [] | |
| Гепсидин и индекс фиброза печени MELD-XI как маркеры полиорганной недостаточности у пациентов хронической сердечной недостаточностью с?… [] | |
| Ассоциации уровней NT-proBNP и гепсидина с клинико-лабораторными параметрами у больных хронической сердечной недостаточностью с разной … [] | |
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