白介素2(IL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Interleukin 2 (IL2)
TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin
- 編號USEA073Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.4pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1588 ¥ 2268 ¥ 10206 ¥ 19278 ¥ 158760
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測白介素2(IL2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素2(IL2)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-99 | 94 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 94-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白介素2(IL2)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素2(IL2)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-97% | 92-105% | 88-96% | 96-103% |
| EDTA plasma(n=5) | 78-102% | 79-90% | 79-94% | 78-92% |
| heparin plasma(n=5) | 95-102% | 86-94% | 86-93% | 90-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將白介素2(IL2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的白介素2(IL2)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的白介素2(IL2)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的白介素2(IL2)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA073Bo01 | 白介素2(IL2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA073Bo01 | 白介素2(IL2)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA073Bo21 | 白介素2(IL2)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA073Bo | 白介素2(IL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA073Bo | 白介素2(IL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA073Bo | 白介素2(IL2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA073Bo01 | 白介素2(IL2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UPGA073Bo01 | 白介素2(IL2)引物對 | PCR |
| UCGA073Bo01 | 白介素2(IL2)cDNA | PCR、蛋白表達(dá)等 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Medicinal Plants Research | Evaluation of water plantain (Alisma canaliculatum A. Br. et Bouche) and mistletoe (Viscum album L.) effects on broiler growth performance, meat composition and serum biochemical parameters [AcadeMic: source] |
| Bone | Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration [PubMed: 22796416] |
| Nanomedicine | Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform [PubMed: 23394132] |
| Food and Chemical Toxicology | Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta [Pubmed:25662032] |
| Pharmaceutical Research | Molecular Mechanisms of the Modulatory Effect of Vitamin E on Tacrolimus (FK506)-Induced Renal Injury in Rats [Openview: 6Ba34F298130121B36Fc3E227Ed416F4] |
| PLoS Neglected Tropical Diseases | Efficacy of Albendazole-Chitosan Microsphere-based Treatment for Alveolar Echinococcosis in Mice [PubMed: 26352932] |
| Int J Clin Exp Med | Berberine alleviates preeclampsia possibly by regulating the expression of interleukin-2/interleukin-10 and Bcl-2/Bax [PubMed: 26629148] |
| Clin Oral Investig | Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? [PubMed: 26556576] |
| veterinary research forum | Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ] |
| Pharmaceutical Biology | Molecular mechanisms of the protective role of wheat germ oil against cyclosporin A-induced hepatotoxicity in rats [PubMed: 25858514] |
| Immunologic Research | Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE10 and is crucial for liver transplant tolerance [PubMed: 25982596] |
| J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
| удк | Циток [Cgi-Bin: Irbis_Nbuv] |
| Biomark Med | Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma [PubMed: 26609634] |
| Environmental Science and Pollution Research | Ameliorative effect of flaxseed oil against thiacloprid-induced toxicity in rats: hematological, biochemical, and histopathological study [Pubmed:26957428] |
| Advances in Experimental Medicine and Biology? | Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury [Pubmed:27283193] |
| Research in Veterinary Science | Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens [Pubmed:27473977] |
| Biomedicine & Pharmacotherapy | Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate [Pubmed:27261592] |
| Int J Pharm Bio Sci | International Journal of Pharma and Bio Sciences ISSN [cms:4987] |
| Asian J Pharm Clin Res | INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790] |
| United States Patent Application | HERPES VIRUS VACCINES AND TREATMENTS [y2016:0067330.html] |
| BMC Veterinary Research | Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis [pubmed:27553600] |
| Vaccine. | Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J. [pubmed:28049588] |
| Taylor & Francis | Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation [09540105.2017.1306494] |
| Physiological?Research | Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. [pubmed:28006947] |
| Journal?of?Virology | Characterization?of?Simian?Immunodeficiency?Virus?Variants?Anatomically?Compartmentalized?inPlasma?and?Milk?in?Chronically?Infected?African?Green?Monkeys. [pubmed:] |
| ?Research in Veterinary | The effect of feed supplementation with zinc chelate and zinc sulphate on selected humoral and cell-mediated immune parameters and cytokine concentration in … [S0034528816303162] |
| Research in Veterinary Science | Commercial gold nanocolloid inhibits synthesis of IL-2 and proliferation of porcine T lymphocytes [science:article] |
| Acta Vet Hung. | Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. [pubmed:28244338] |
| Домашня стор?нка | ВПЛИВ КРОВОВТРАТИ НА ДИНАМ?КУ ВМ?СТУ ЦИТОК?Н?В СИРОВАТКИ КРОВ? В ПЕР?ОД ГОСТРО? РЕАКЦ?? НА КРАН?ОСКЕЛЕТНУ ТРАВМУ [83646] |
| Journal of Agricultural and Food Chemistry | Effect of boron on thymic cytokine expression, hormone secretion, antioxidant functions, cell proliferation, and apoptosis potential via the ERK1/2 signaling pathway [pubmed:29032684] |
| Research in Veterinary Science | The effect of feed supplementation with effective microorganisms(EM) on pro- and anti-inflammatory cytokine concentrations in pigs [pubmed:28549299] |
| poultry?science | The effect of feed supplementation with Zakarpacki zeolite (clinoptilolite) on percentages of T and B lymphocytes and cytokine concentrations in poultry [pubmed:28339915] |
| Journal of Animal Physiology and Animal Nutrition | The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens [doi:10.1111] |
| Molecular Medicine Reports | Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury [10.3892/mmr.2017.6837] |
| Veterinary Microbiology | A bacterial ghost improves the immunological efficacy of a Newcastle disease virus inactivated vaccine [pubmed:28619143] |
| Canadian Journal of Gastroenterology and Hepatology | EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-?傠Signaling Pathway in Rats [10.1155/2017/3057268] |
| Veterinary Immunology and Immunopathology | Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. [pubmed:28895861] |
| Biological Rhythm Research | A study of altered cytokine rhythms associated with successful implantation in cows [10.1080/09291016.2017.1361159] |
| Journal of Pain Research | Sex differences in complex regional pain syndrome type I (CRPS-I) in mice [pubmed:28831269] |
| Canadian Journal of Gastroenterology and Hepatology | EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-κB Signaling Pathway in Rats [10.1155/2017/3057268] |
| In Vivo Study on the Hindrance Activity of Cinnamon Extract Against Aspergillus niger in Mice [ISSN: 0974-4290] | |
| Cancer?Science | Sonodynamic therapy‐assisted immunotherapy: A novel modality for cancer treatment [Pubmed:29575297] |
| PLoS One | Cyclosporine a drug-delivery system for high-risk penetrating keratoplasty: Stabilizing the intraocular immune microenvironment [Pubmed:29734357] |
| Probiotics Antimicrob Proteins | Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a?… [Pubmed:29305686] |
| Microbial Pathogenesis | Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos?… [Pubmed:29787791] |
| Theriogenology | Effect of tropical thermal stress on peri-implantation immune responses in cows [Pubmed:29625402] |
| Food?&?Nutrition?Research | Immunomodulatory activity of Lactobacillus plantarum KLDS1. 0318 in cyclophosphamide-treated mice [Pubmed:30026678] |
| Journal of?Animal?Physiology and?Animal?Nutrition | The effect of feed supplementation with a copper‐glycine chelate and copper sulphate on selected humoral and cell‐mediated immune parameters, plasma?… [Pubmed:28603872] |
| Journal of?International?Medical?Research | Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127] |
| International Journal of Environmental Studies? | Effectiveness of steamed and cooked broccoli to attenuate bone marrow injury and suppressed haemopoiesis in male rats exposed to petrol vapours [10.1080:00207233.2018.1502956] |
| Veterinary?Immunology?and?Immunopathology | Evaluation of T regulatory lymphocytes and serum concentration of selected cytokines in dogs with perianal tumors [] |
| International?Immunopharmacology | Vitamin E inhibits cyclosporin A-induced CTGF and TIMP-1 expression by repressing ROS-mediated activation of TGF-β/Smad signaling pathway in rat liver [Pubmed: 30391882] |
| Journal of Veterinary?Research | Early cytokine response after vaccination with Coxiella burnetii phase I in an infected herd of dairy cattle [] |
| Journal of?Neuroinflammation | Amlexanox attenuates experimental autoimmune encephalomyelitis by inhibiting dendritic cell maturation and reprogramming effector and regulatory T cell?… [Pubmed: 30823934] |
| International?Journal?of?Laboratory?Hematology | CD56 natural killer cells exhibit abnormal phenotype and function in severe aplastic anemia [] |
| International Immunopharmacology | Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c mice [Pubmed: 30974284] |
| 中國免疫學(xué)雜志 | TRAF-6 調(diào)控TGF-β1-Smad2/Smad3 信號通路在Graves 病免疫發(fā)病機制中的作用 [] |
| Animals | Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training [Pubmed: 31462005] |
| Journal of Medicinal Food | Polysaccharide from the Seeds of Plantago asiatica L. Protect Against Lipopolysaccharide-Induced Liver Injury [Pubmed: 31560611] |
| international urology and?nephrology | The therapeutic effect of dendritic cells expressing indoleamine 2, 3-dioxygenase (IDO) on an IgA nephropathy mouse model [Pubmed: 31894556] |
| Transboundary and Emerging Diseases | Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China [Pubmed: 32187856] |
| Nanomedicine | The toxicity of alumina nanoparticles to immune system in mice [Pubmed: 32162999] |
| Immunopharmacol Immunotoxicol | Cordycepin exhibits a suppressive effect on T cells through inhibiting TCR signaling cascade in CFA-induced inflammation mice model [Pubmed: 32105161] |
| Journal of Receptors and Signal Transduction Research | Suppressor of ras val-2 promotes inflammation-mediated oxidative stress and cell apoptosis in cardiomyocytes through activating Mst1-mROS signaling pathway [Pubmed: 32065019] |
| Antibiotics | Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal Pigs [Pubmed: 31963653] |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705] |
| JOURNAL OF CHEMOTHERAPY | Active metronomic vinorelbine schedules decrease plasma interleukin-2 levels in mice with Lewis lung carcinoma [Pubmed: 32930084] |
| THERIOGENOLOGY | Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion [Pubmed: 32810792] |
| Theriogenology | A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows [Pubmed: 32810791] |
| Life Sci | Impact of diminazene aceturate on renin-angiotensin system, infectious myocarditis and skeletal myositis in mice: An in vitro and in vivo study [Pubmed: 32652140] |
| BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225] |
| IMMUNOSTIMULATORY EFFECT OF MORINGA OLEIFERA LEAVES EXTRACT ON CYCLOPHOSPHAMIDE-INDUCED IMMUNOSUPPRESSION IN MICE [] | |
| ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction [] |
| Immunomodulatory effects of sCD83 via induction of TGF-β1 and IDO: an in vitro and rat orthotopic liver transplantation study [] | |
| Hormonal and Cytokine Blood Profile of Dairy Cows in the Early Gestation Period [] | |
| THE CHANGE OF INDICATORS OF PROTEIN, LIPID AND CARBOHYDRATE METABOLISM IN DAIRY COWS AFTER CALVING DURING THE FORMATION OF?… [] | |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512] |
| Eur Rev Med Pharmacol Sci | Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients [33755963] |
| Dig Dis Sci | Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway [34097168] |
| How Does the Th1/Th2 Polarization in Milk Change According to the Types of Bacteria that Cause Subclinical Mastitis in Cows? [] | |
| World J Tradit Chin Med | Experimental autoimmune encephalomyelitis inhibited by Huangqi Guizhi Wuwu Decoction via Th2 cytokine enhancement [] |
| Journal of Herbs, Spices & Medicinal Plants | Immunostimulatory Effect of Moringa oleifera Extracts on Cyclophosphamide-induced Immunosuppressed Mice [] |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237] |
| J Immunol Res | Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus [34395633] |
| Experimental and Therapeutic Medicine | Autonomic regulation of imbalance?induced myocardial fibrosis and its mechanism in rats with cirrhosis [34373726] |
| Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice [34960627] |
| Animal Nutrition | Optimizing the growth and immune system of dairy calves by subdividing the pre-weaning period and providing different milk volumes for each stage [34786502] |
| Cancer Commun (Lond) | Anti‐PD‐L1/TGF‐βR fusion protein (SHR‐1701) overcomes disrupted lymphocyte recovery‐induced resistance to PD‐1/PD‐L1 inhibitors in lung cancer [Pubmed:34981670] |
| Int J Mol Sci | NLRP3 Inflammasome Activation Controls Vascular Smooth Muscle Cells Phenotypic Switch in Atherosclerosis [Pubmed:35008765] |
| Scientific Reports | A randomized controlled trial evaluating the effect of two low-level laser irradiation protocols on the rate of canine retraction [Pubmed:35710926] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
| Toxicology Research | Alleviation of chemotherapy-induced acute lung injury via NLRP3/ASC/Caspase-1 signaling pathway [Pubmed:35782645] |
| Vaccines | Age-Dependent Dynamics of Maternally Derived Antibodies (MDAs) and Understanding MDA-Mediated Immune Tolerance in Foot-and-Mouth Disease-Vaccinated?… [Pubmed:35632433] |
| Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows [] |
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