胰島素(INS)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Insulin (INS)
- 編號(hào)UKSA448Mu11
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍123.5-10000pg/mL
- 可適靈敏度52.4pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3499 ¥ 5832 ¥ 10498 ¥ 20412 ¥ 34992
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)胰島素 (INS)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA448Mu02 | 胰島素(INS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA448Mu01 | 胰島素(INS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA448Mu01 | 胰島素(INS)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA448Mu02 | 胰島素(INS)多克隆抗體 | WB; IHC |
| UPAA448Mu01 | 胰島素(INS)多克隆抗體 | WB; IHC; ICC/IF |
| UMEA448Mu | 胰島素(INS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA448Mu | 胰島素(INS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA448Mu | 胰島素(INS)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA448Mu11 | 胰島素(INS)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Surgical Endoscopy and Other Interventional Techniques | Distal gastric mucosa ablation induces significant weight loss and improved glycemic control in type 2 diabetes Sprague–Dawley rat model [Pubmed: 31630290] |
| Biomedicine & Pharmacotherapy | Propolis modulates the gut microbiota and improves the intestinal mucosal barrier function in diabetic rats [Pubmed: 31545258] |
| International Journal of Endocrinology | miR-29a Negatively Affects Glucose-Stimulated Insulin Secretion and MIN6 Cell Proliferation via Cdc42/β-Catenin Signaling [Pubmed: 31662747] |
| Food & Function | Depolymerized RG-I enriched pectin from citrus segment membrane modulates gut microbiota, increases SCFAs production, promotes the growth of Bifidobacterium?… [Pubmed: 31778135] |
| Life Sciences | P-Coumaric acid alleviates experimental diabetic nephropathy through modulation of toll like receptor-4 in rats [Pubmed: 31629762] |
| Journal of Ovarian Research | Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718] |
| journal of ethnopharmacology | Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig model [Pubmed: 31756448] |
| Brain Research | Isoflurane aggravates peripheral and central insulin resistance in high-fat diet/streptozocin-induced type 2 diabetic mice [Pubmed: 31672472] |
| Journal of Ethnopharmacology | San-Huang-Tang protects obesity/diabetes induced NAFLD by upregulating PGC-1α/PEPCK signaling in obese and galr1 knockout mice models [Pubmed: 31843573] |
| Journal of Hazardous Materials | Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal mice [Pubmed: 31796355] |
| Journal of Steroid Biochemistry and Molecular Biology | SerpinB1 promotes the proliferation of porcine pancreatic stem cells through the STAT3 signaling pathway [Pubmed: 31785377] |
| Journal of dermatological treatment | Liraglutide improved inflammation via mediating IL-23/Th-17 pathway in obese diabetic mice with psoriasiform skin [Pubmed: 31868553] |
| Nutrients | Rotundic Acid Protects against Metabolic Disturbance and Improves Gut Microbiota in Type 2 Diabetes Rats [Pubmed: 31887996] |
| JOURNAL OF DAIRY SCIENCE | Decreased amylolytic microbes of the hindgut and increased blood glucose implied improved starch utilization in the small intestine by using rumen-protected leucine … [Pubmed: 32113753] |
| Biomed Pharmacother | Composite probiotics alleviate type 2 diabetes by regulating intestinal microbiota and inducing GLP-1 secretion in db/db mice [Pubmed: 32035395] |
| Frontiers in Endocrinology | Hyperbaric oxygen ameliorates insulin sensitivity by increasing GLUT4 expression in skeletal muscle and stimulating UCP1 in brown adipose tissue in T2DM … [Pubmed: 32082261] |
| Cell Physiol Biochem | Integrin Linked Kinase (ILK) Downregulation as an Early Event During the Development of Metabolic Alterations in a Short-Term High Fat Diet Mice Model. [Pubmed: 31972071] |
| JOURNAL OF FOOD SCIENCE | Beneficial effects of Aronia melanocarpa berry extract on hepatic insulin resistance in type 2 diabetes mellitus rats [Pubmed: 32249934] |
| Journal of Diabetes Research | Roux-en-Y Gastric Bypass in Obese Diabetic Rats Promotes Autophagy to Improve Lipid Metabolism through mTOR/p70S6K Signaling Pathway [Pubmed: 32309446] |
| Brain Research | Electroacupuncture improves cognitive deficits and insulin resistance in an OLETF rat model of Al/D-gal induced aging model via the PI3K/Akt signaling pathway [Pubmed: 32304687] |
| endocrine journal | Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose?… [Pubmed: 32249244] |
| Frontiers in Endocrinology | RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis [Pubmed: 32328034] |
| Antioxidants | Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to Diabetes [Pubmed: 33203103] |
| Nature Communications | AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axis [Pubmed: 33199780] |
| EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | Evaluation of the hypoglycemic effect of exendin-4's new oral self-nanoemulsifying system in rats [Pubmed: 33197556] |
| BIOLOGICAL RESEARCH | Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance?… [Pubmed: 33109277] |
| FASEB JOURNAL | Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and?… [Pubmed: 32954574] |
| Biol Res | Palmatine ameliorates high fat diet induced impaired glucose tolerance [Pubmed: 32928312] |
| ENDOCRINE | Thioredoxin-interacting protein: a critical link between autophagy disorders and pancreatic β-cell dysfunction [Pubmed: 32892310] |
| DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via?… [Pubmed: 32880686] |
| AMERICAN JOURNAL OF PATHOLOGY | Insulin promotes corneal nerve repair and wound healing in type 1 diabetic mice by enhancing Wnt/β-catenin signaling [Pubmed: 32858016] |
| MEDIATORS OF INFLAMMATION | Aplysin Retards Pancreatic Necrosis and Inflammatory Responses in NOD Mice by Stabilizing Intestinal Barriers and Regulating Gut Microbial Composition [Pubmed: 32801992] |
| EUROPEAN JOURNAL OF PHARMACOLOGY | Gut microbiota modulation as a promising therapy with metformin in rats with non-alcoholic steatohepatitis: Role of LPS/TLR4 and autophagy pathways [Pubmed: 32758573] |
| ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043] |
| CHEMICO-BIOLOGICAL INTERACTIONS | Modulatory role of imatinib mesylate on pancreatic β-cells' secretory functions in an STZ rat model of diabetes mellitus [Pubmed: 32710900] |
| Mol Med | Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic?… [Pubmed: 32698821] |
| JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity [Pubmed: 32633953] |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
| J Endocrinol Invest | Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic rats [Pubmed: 32588381] |
| J Chem Neuroanat | The neuroprotective effect of simvastatin on the cerebellum of experimentally-induced diabetic rats through klotho upregulation: An immunohistochemical study [Pubmed: 32479899] |
| Journal of Diabetes Research | Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus [Pubmed: 33123597] |
| Journal of Cellular and Molecular Medicine | Cooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic rats [Pubmed: 32395890] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| Frontiers in Pharmacology | MiR-185-5p Protects Against Angiogenesis in Polycystic Ovary Syndrome by Targeting VEGFA [Pubmed: 32760272] |
| Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS [] |
| Nutrition & Metabolism | Fucoidan prevent murine autoimmune diabetes via suppression TLR4-signaling pathways, regulation DC/Treg induced immune tolerance and improving gut?… [] |
| Journal of Functional Foods | Different source of commercial vegetable oils may regulate metabolic, inflammatory and redox status in healthy rats [] |
| Comparative transcriptomic and metabolomic analysis reveals pectoralis highland adaptation across altitudinal songbirds [] | |
| The effects of different intensity endurance and resistance exercise on diabetic-related blood profiles in impaired glucose tolerance mice [] | |
| Cell Death & Disease | Phosphorylation of eIF2|á signaling pathway attenuates obesity-induced non-alcoholic fatty liver disease in an ER stress and autophagy-dependent manner [33318479] |
| Ther Adv Chronic Dis | Empagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in mice [33294147] |
| Physiol Behav | Time-restricted feeding attenuates gluconeogenic activity through inhibition of PGC-1|á expression and activity [33412190] |
| PLoS Genetics | Physiological and genetic convergence supports hypoxia resistance in high-altitude songbirds [33370292] |
| Mol Med Rep | N1?methylnicotinamide ameliorates insulin resistance in skeletal muscle of type 2 diabetic mice by activating the SIRT1/PGC?1|á signaling pathway [33576435] |
| Arch Endocrinol Metab | Inverse association of plasma hydrogen sulfide levels with visceral fat area among Chinese young men: a cross-sectional study [33740335] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| PeerJ | miR-155-5p upregulation ameliorates myocardial insulin resistance via mTOR signaling in chronic alcohol drinking rats [33868799] |
| JOURNAL OF FOOD BIOCHEMISTRY | Identification of curcumin as a potential |á?\glucosidase and dipeptidyl?\peptidase 4 inhibitor: Molecular docking study, in vitro and in vivo biological evaluation [33817806] |
| JOURNAL OF NUTRITIONAL BIOCHEMISTRY | Consumption of soybean or olive oil at recommended concentrations increased the intestinal microbiota diversity and insulin sensitivity and prevented fatty liver??- [33915261] |
| Cell Metab | TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease [34107313] |
| Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF|?1/Smad ?- [34116674] | |
| EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY | Different Ratios of Corn and Coconut Oil Blends in High?\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats [] |
| Journal of Alzheimer's disease | DAla2-GIP-GLU-PAL Protects Against Cognitive Deficits and Pathology in APP/PS1 Mice by Inhibiting Neuroinflammation and Up-regulating cAMP/PKA/CREB??- [] |
| International Journal of Diabetes in Developing Countries | Investigation of extracellular matrix genes associated with Alzheimer's disease in the hippocampus of experimental diabetic model rats [] |
| researchsquare | Ficus deltoidea ameliorates biochemical, hormonal, and histomorphometric changes in letrozole-induced polycystic ovarian syndrome rats [] |
| Cells Tissues Organs | Intermittent Fasting Attenuates High Fat Diet-Induced Cerebellar Changes in Rats: Involvement of TNFα, autophagy and oxidative stress [34551416] |
| Experimental Biology and Medicine | Bee venom ameliorates cardiac dysfunction in diabetic hyperlipidemic rats [34550826] |
| Phytomedicine | Baicalin protects against insulin resistance and metabolic dysfunction through activation of GALR2/GLUT4 signaling [34923235] |
| Pharmacol Res | Lactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeability [34896249] |
| Eat Weight Disord | Time-restricted feeding prevents metabolic diseases through the regulation of galanin/GALR1 expression in the hypothalamus of mice [34370270] |
| Food Chem Toxicol | Combined treatment of sinapic acid and ellagic acid attenuates hyperglycemia in streptozotocin-induced diabetic rats [34329744] |
| Eur J Pharmacol | Spexin ameliorates skeletal muscle insulin resistance through activation of GAL2 receptor [Pubmed:34973950] |
| Surgical Endoscopy | The effect of bariatric surgery on the expression of Growth Differentiation Factor-15/Macrophage-Inhibitory Cytokine-1 (GDF-15/MIC-1) in rat [Pubmed:35029766] |
| Arch Biochem Biophys | ATP6V1H deficiency impairs glucose tolerance by augmenting endoplasmic reticulum stress in high fat diet fed mice [Pubmed:34990584] |
| Environ Sci Pollut Res Int | Environmental exposure to low-dose perfluorohexanesulfonate promotes obesity and non-alcoholic fatty liver disease in mice fed a high-fat diet [Pubmed:35217953] |
| Naunyn Schmiedebergs Arch Pharmacol | Intragastric safflower yellow and its main component HSYA improve leptin sensitivity before body weight change in diet-induced obese mice [Pubmed:35201390] |
| PLoS One | A cross-sectional comparative study on the effects of body mass index and exercise/sedentary on serum asprosin in male college students [Pubmed:35381008] |
| Cell Death & Disease | Hepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistance [Pubmed:35347118] |
| Endocr J | A novel anti-obesity mechanism for liraglutide by improving adipose tissue leptin resistance in high-fat diet-fed obese mice [Pubmed:35705299] |
| Antioxidants | Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice [Pubmed:35740008] |
| Mol Cell Endocrinol | Treatment with spexin mitigates diet-induced hepatic steatosis in vivo and in vitro through activation of galanin receptor 2 [Pubmed:35654225] |
| Biofabrication | Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells [Pubmed:35616388] |
| Ecotoxicology and Environmental Safety | Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in utero [Pubmed:35679725] |
| SAUDI J BIOL SCI | Aqueous Ajwa dates seeds extract improves memory impairment in type-2 diabetes mellitus rats by reducing blood glucose levels and enhancing brain?… [Pubmed:35531250] |
| Saudi Pharmaceutical Journal | Sukkari dates seed improves type-2 diabetes mellitus-induced memory impairment by reducing blood glucose levels and enhancing brain cholinergic?… [Pubmed:35812141] |
| Turk J Diab Obes | An Investigation of the Effect of Curcumin on Fructose-Induced Metabolic Syndrome Rat Models [] |
| Journal of Drug Delivery Science and Technology | Miglitol-PMO nanoparticles, an alpha-glucosidase inhibitor, has prolonged action on blood glucose level in diabetic rats [] |
| Genes & Diseases | Prolonged control of insulin-dependent diabetes via intramuscular expression of plasmid-encoded single-strand insulin analogue [] |
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