神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體
Polyclonal Antibody to Enolase, Neuron Specific (NSE)
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
- 編號UPAA537Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA537Hu01-神經(jīng)元特異性烯醇化酶(NSE)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 284 ¥ 664 ¥ 948 ¥ 2370 ¥ 9480
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 10ug/ml Rabbit Anti-Human NSE Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Cerebrum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human NSE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Sample: Porcine Cerebrum lysate Primary Ab: 0.05μg/ml Rabbit Anti-Human NSE Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: HepG2 cell lysate; Lane4: 293T cell lysate;
Primary Ab: 0.5μg/ml Rabbit Anti-Human NSE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification: <br/>Lane 1: Wild-type HepG2 cell lysate; <br/>Lane 2: NSE knockout HepG2 cell lysate; <br/>Predicted MW: 43,48kDa <br/>Observed MW: 50kDa<br/>Primary Ab: 2μg/ml Rabbit Anti-Human NSE Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western Blot; Sample: Fetal Rat Cerebrum lysate
Primary Ab: 0.05μg/ml Rabbit Anti-Human NSE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: A549 cell lysate; Lane3: SH-SY5Y cell lysate; Lane4: U87MG cell lysate;
Primary Ab: 0.05μg/ml Rabbit Anti-Human NSE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對NSE的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別NSE。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
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| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA537Hu61 | 神經(jīng)元特異性烯醇化酶(NSE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA537Hu02 | 神經(jīng)元特異性烯醇化酶(NSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA537Hu01 | 神經(jīng)元特異性烯醇化酶(NSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA537Hu01 | 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體 | WB; IHC |
| UPAA537Hu02 | 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體 | WB |
| ULAA537Hu71 | 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體(生物素標記) | WB |
| ULAA537Hu81 | 神經(jīng)元特異性烯醇化酶(NSE)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAA537Hu27 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB |
| UMAA537Hu29 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu25 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu21 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; ICC/IF |
| UMAA537Hu22 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA537Hu23 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA537Hu26 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu28 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; ICC/IF |
| UMAA537Hu24 | 神經(jīng)元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| USEA537Hu | 神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA537Hu | 神經(jīng)元特異性烯醇化酶(NSE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA537Hu01 | 神經(jīng)元特異性烯醇化酶(NSE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UCGA537Hu01 | 神經(jīng)元特異性烯醇化酶(NSE)cDNA | PCR、蛋白表達等 |
參考文獻
| 雜志 | 參考文獻 |
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| Int J Neurosci. | The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247] |
| Experimental & Molecular Medicine | Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349] |
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| Physiol Rep | S100B and NSE serum concentrations after simulated diving in rats [PubMed: 26462746] |
| Int J Mol Sci | The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions [PubMed: 26184156] |
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| Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830] |
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| Biomed?Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336] |
| Journal of the Neurological?Sciences | S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid?… [Pubmed:29801873] |
| Iranian Journal of Veterinary Research | Nitrosative stress indices in dogs with neurological form of canine distemper [Doi: 10.22099/IJVR.2018.4943] |
| Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway [] |
| The?Eurasian?Journal?of?Medicine | Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema [] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the?… [] |
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| European Review for Medical and Pharmacological Sciences | Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … [Pubmed: 32432773] |
| Neurobiology of Learning and Memory | Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats [] |
| The role of light desynchronosis in the development of stress-induced aging [] | |
| ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ?… [] | |
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| Ther Adv Chronic Dis | The effect of doxycycline on neuron-specific enolase in patients with traumatic brain injury: a randomized controlled trial [34262678] |
| J Surg Res | Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway [34399353] |
| Cephalalgia | Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome [34644195] |
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