白介素5(IL5)活性蛋白
Active Interleukin 5 (IL5)
E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor
- 編號(hào)UAPA078Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)7.0
- 應(yīng)用Cell culture; Activity Assays; In vivo assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1058 ¥ 2646 ¥ 5292 ¥ 15876 ¥ 39690
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Interleukin-5 (IL-5), a secreted glycoprotein, is a member of the hematopoietic family. Interleukin 5 has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104cells/well and incubated for 48h in the presence or absence of various concentrations of IL-5 at 37oC. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.
Cell proliferation of TF-1 cells after incubation with IL-5 for 48h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of IL-5 was shown in Figure 2. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 0.18 to 7.675ng/mL.
用法
Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA078Hu61 | 白介素5(IL5)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA078Hu01 | 白介素5(IL5)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA078Hu62 | 白介素5(IL5)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA078Hu62 | 白介素5(IL5)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA078Hu61 | 白介素5(IL5)活性蛋白 | Cell culture; Activity Assays; In vivo assays. |
| UEPA078Hu41 | 白介素5(IL5)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA078Hu01 | 白介素5(IL5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA078Hu01 | 白介素5(IL5)多克隆抗體 | IHC; FCM |
| UFAA078Hu01 | 抗白介素5(IL5)多克隆抗體 | FCM |
| URAA078Hu21 | 白介素5(IL5)重組抗體 | FCM |
| UMAA078Hu21 | 白介素5(IL5)單克隆抗體 | WB |
| UMAA078Hu22 | 白介素5(IL5)單克隆抗體 | WB |
| USEA078Hu | 白介素5(IL5)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA078Hu | 白介素5(IL5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UIGA078Hu21 | 白介素5(IL5)人基因嵌入小鼠 | 動(dòng)物實(shí)驗(yàn)，陽性對(duì)照。 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Critical Care Medicine | Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056] |
| veterinary research forum | Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells [Article_14936_6.Html: ] |
| Molecular Immunology | Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-??B in murine model of asthma [Pubmed:27289030] |
| Advances in Experimental Medicine and Biology | Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Cavia (Guinea pig )inea Pigs [Pubmed:27130219] |
| Sci Rep. | Interleukin-31 expression and relation to disease severity in human asthma [pmc:PMC4783779] |
| Molecular?Immunology | Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma [pubmed:27289030] |
| Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
| Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
| Acta?Pharmacologica Sinica | Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi') [Pubmed: 19363516] |
| International Journal of Peptide Research and Therapeutics | ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma [] |
| Journal of?Pineal?Research | Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267] |
| Analytica Chimica Acta | An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis [] |
| American Journal of Translational Research | Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193] |
| bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767] |
| Biomedicine & Pharmacotherapy | Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice [Pubmed: 32521453] |
| Journal of Traditional Chinese Medical Sciences | Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice [] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota [] | |
| Annals of Tropical Medicine & Public Health | Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq [] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| Mol Immunol | MiR-142-5p directly targets cyclin-dependent kinase 5-mediated upregulation of the inflammatory process in acquired middle ear cholesteatoma [34875451] |
| Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows [] |
