白介素4(IL4)活性蛋白
Active Interleukin 4 (IL4)
BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
- 編號UAPA077Ov01
- 物種Ovis aries; Ovine (Sheep，綿羊) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)9.4
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 960 ¥ 2400 ¥ 4800 ¥ 14400 ¥ 36000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells.It plays vital biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells and monocyte. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. To test the effect of sheep IL4 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant sheep IL4. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant sheep IL4 for 72h. The ED50 of recombinant sheep IL4 is 0.077 μg/ml.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Ovis aries; Ovine (Sheep，綿羊) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA077Ov01 | 白介素4(IL4)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA077Ov01 | 白介素4(IL4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA077Ov61 | 白介素4(IL4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA077Ov01 | 白介素4(IL4)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAA077Ov21 | 白介素4(IL4)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA077Ov | 白介素4(IL4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA077Ov | 白介素4(IL4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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