白介素11(IL11)活性蛋白
Active Interleukin 11 (IL11)
AGIF; Adipogenesis Inhibitory Factor; Oprelvekin
- 編號UAPA057Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點11.7
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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Sample: Recombinant IL11, Human;
Antibody: Rabbit Anti-Human IL11 Ab (PAA057Hu06)
Figure. Western Blot
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Interleukin 11 (IL-11) is a multifunctional cytokine. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation IL-11 causes bone-resorption. Besides, IL-11 have been proved can promote migration of A549 cells, 5×104 cells were seeded into 6 well plates. After cell confluent, replace with DMEM without serum overnight. Using a (yellow) pipette tip make a straight scratch, simulating a wound, then washing the wells three times with PBS. Adding 1% serum standard DMEM containing various concentrations of recombinan human IL-11 to each well, incubating the plate for 48 hours at 37℃, 5% CO2. The results observed by inverted microscope was shown in Figure.
(A) A549 cells cultured in DMEM with 100ng/mL IL-11 for 48h;
(B) A549 cells cultured in DMEM before addition IL-11.
Figure. Wound healing assay of A549 cells after stimulated with IL-11.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UEPA057Hu61 | 白介素11(IL11)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA057Hu01 | 白介素11(IL11)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA057Hu62 | 白介素11(IL11)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA057Hu01 | 白介素11(IL11)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA057Hu62 | 白介素11(IL11)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA057Hu61 | 白介素11(IL11)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA057Hu01 | 白介素11(IL11)多克隆抗體 | WB |
| UMAA057Hu22 | 白介素11(IL11)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA057Hu21 | 白介素11(IL11)單克隆抗體 | WB |
| USEA057Hu | 白介素11(IL11)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA057Hu | 白介素11(IL11)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Molecular Medicine Reports | Regulatory effect of chemerin and therapeutic efficacy of chemerin?9 in pancreatogenic diabetes mellitus [Pubmed: 31922216] |
